Single-Cell and Spatial Genomics Platform
Since 2019, CTG has offered a broad range of single-cell applications, providing expert knowledge, accessibility, and personalized support. We continuously update our methods and instruments in collaboration with local researchers, ensuring that we stay in tune with the evolving needs of the research community. If a critical method is missing from our offerings, please don't hesitate to reach out to us.
General guidelines
Consultations and Service Projects, as well as a Booking Schedule are available in iLab (see “How to start a project”)
Gentle sample isolation is essential to prevent bias against vulnerable cell types while fast isolation is desirable to reduce degradation of nucleic acids.
Thus, prior to initiating a project at the Single-Cell Genomics Platform, users are asked to thoroughly evaluate a cell or nucleus isolation strategy suitable for their target tissue and research question. Users are also strongly encouraged to evaluate their sample quality (e.g. RNA integrity) prior to submitting single-cell genomics work at the facility.
Once a project is accepted by the Single-Cell Genomics Platform, users must follow sample preparation and delivery instructions provided by the platform.
Available services
scTranscriptomics
- Universal 3' Gene Expression (10x Genomics, v4)
- Hashing
- Cell Surface Protein
- Universal 5' Gene Expression (10x Genomics, v3)
- Full-length TCR/BCR sequences
- Hashing
- Cell Surface Protein
- CRISPR Screen
- Epi Multiome (10x Genomics)
scTranscriptomics (fixed cells/nuclei)
- Flex Gene Expression (10x Genomics, v2 and v3)
- Cell Surface / Intracellular Protein
- Custom probe design
- CRISPR Screen
- Evercode™ WT (Parse Bioscience)
scEpigenomics
- Epi ATAC (10x Genomics, v2)
- Epi Multiome (10x Genomics)
Cell isolation
- High precision cell isolation of sparse biological samples using CellenOne F1.4
Spatial transcriptomics
- Visium or Visium HD for FFPE Fixed specimens
Equipment
- 10x Genomics Chromium X
- 10x Genomics Visium CytAssist
- Scienion CellenOne F1.4
- Nexcelom Cellaca Mx
- Illumina Sequencer NextSeq 2000, NovaSeq X Plus
Applications under development
- Single-cell proteomics using the proteochip on the CellenOne F1.4.
- Customization of existing 10x Genomics applications (e.g. DogmaSeq, viral barcode capturing, integration with long-read technology using Oxford Nanopore).
If you are interested in collaborating with us for our protocols under development, or if you have a suggestion for the implementation or development of a novel method, please contact Julia Bräunig (singlecell [dot] ctgservice [at] med [dot] lu [dot] se (singlecell[dot]ctgservice[at]med[dot]lu[dot]se)).
Special Offers for New Users!
New to 10x Genomics?
Get 50% off 4rxn kits for Universal, Flex, and Epi in Q1 2025.
Trying Parse Evercode for the first time?
Enjoy up to 45% off your first run:
- Mini: 45% off
- WT: 18% off
- Mega 96: 8% off
- Mega 384: 5% off
Requests for service/consults:
Link to iLab
Contact Information
ctg_service [at] med [dot] lu [dot] se
singlecell [dot] ctgservice [at] med [dot] lu [dot] se
Phone
+46 73 075 3563
+46 73 096 8048
Address
BMC - Biomedical Centre D14
Klinikgatan 32
221 85 Lund